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The current challenge in agriculture is to increase crop yields while maintaining sustainability. Precision agriculture, genomics, and synthetic biology have made significant advancements in achieving global food security and environmental sustainability. Precision agriculture uses real-time data to optimize crop management strategies. Artificial intelligence and machine learning help in data-driven decision-making and disease identification. High-throughput sequencing allows for faster examination of plant genomes and understanding genetic foundations. Genome-editing methods like CRISPR-Cas9 enable precise alterations in crop genomes. Synthetic biology aims to boost agricultural productivity through the design and manipulation of biological elements. It is important to study modern agricultural biotechnology tools in this context. We currently find ourselves at a critical juncture in the trajectory of agricultural production within the annals of history. The challenge is to enhance crop yields while incorporating appropriate levels of sustainability built within the production systems. The pursuit of global food security and environmental sustainability has spurred notable progress in agricultural technologies such as precision agriculture, genomics, and synthetic biology. Precision agriculture leverages real-time data from sensors, drones, satellites in order to enhance the efficiency and effectiveness of crop management strategies enabling the farmers to optimize resource efficiency and reduce environmental impact. Furthermore, the incorporation of artificial intelligence and machine learning enables the utilization of data-driven decision-making processes, hence enabling the timely identification of diseases and prediction of yield patterns. The introduction of high-throughput sequencing has facilitated expeditious and economically viable examination of plant genomes, leading to elucidation of the underlying genetic foundations of favorable characteristics. Genome-editing methodologies, particularly CRISPR-Cas9, are facilitating the precise and focused alterations of crop genomes. Synthetic biology presents a transformative change through the deliberate design and manipulation of biological elements to boost agricultural productivity. This encompasses the advancement of nitrogen-fixing plants, crops that are resilient to climate extremes, and biofortified types that include enhanced micronutrient contents. Now with all this in background, it becomes all the more important to study the tools of modern-day biotechnology or agricultural biotechnology as we may know it.