The audio titled "Application 1 from ISA" begins with the sound of applause, signifying a crowd's approval or excitement. This is followed by the rhythmic clapping of hands, adding to the atmosphere of enthusiastic support and anticipation. The remainder of the audio is characterized by the respectful and considerate exchanges, evoking a sense of politeness. The audio concludes with a final round of applause and clapping, wrapping up the event or segment with a positive and uplifting note.