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The life cycle of the gliofilary has three stages: cyst, trophozoite, and flagellated. The cyst stage is dormant and occurs during unstable living conditions. It changes to the trophozoite stage during desirable conditions. The trophozoite stage is the feeding stage and replicates in the nasal cavity. The flagellated stage is transitional and is formed due to changes in ionic strength and fluid. The gliofilary is found in warmer freshwater bodies. Both cyst and trophozoite stages can be contracted through infected freshwater entering the nasal cavity. Once in the body, the gliofilary migrates to the brain, causing primary amoebic meningioencephalitis. Symptoms occur during this stage and death usually occurs within 7 to 10 days. Hey, my name is Jesse Yang, and I'm going to talk about the life cycle of the gliofilary. We have three stages. We have the cyst stage, trophozoite stage, and flagellated stage. During the cyst stage, also known as the dormant stage, is the first stage, and it is the most prevalent during unstable living conditions with no set time span. It changes usually to the trophozoite stage during desirable conditions such as the warmer temperatures. During the trophozoite stage, also known as the feeding stage, is infected for phase for humans. It replicates through promitosis in the nasal cavity. And the flagellated stage, which is usually the transitional stage, is usually formed due to being exposed to changes in ionic strength and fluid. The gliofilary is typically found in warmer freshwater bodies such as lakes, rivers, and hot springs. Both cyst and trophozoite stage can be contracted through infected freshwater. The gliofilary can be contracted in both cyst and trophozoite stage. Contraction of the gliofilary is usually by freshwater entering through the passage of the nasal cavity. Once the gliofilary penetrates the nasal mucosa, it will migrate to the brain via olfactory nerves, causing primary amoebic meningioencephalitis, which is usually called PAM. During this stage is where most of the symptoms start occurring, and within 7 to 10 days, the person will die. And thus, once the person dies, it repeats the cycle all over again.